Answer the following question using the databases and search tools at NCBI : Wha
ID: 3863832 • Letter: A
Question
Answer the following question using the databases and search tools at NCBI: What three genes in C. elegans encode proteins very similar in sequence to human pancreatic elastase 1? To make the task of answering the question easier, note the following.
Consider the protein's full name to be "Chymotrypsin-like elastase family member 1".
Do not consider any isoforms of the human protein.
Use a tblastn search.
Search against the "nr" database for C. elegans.
Use the default BLAST search parameters as determined by NCBI.
Read relevant sections of the "Entrez Help Document" and the BLAST Help documentation.
Do not consider any hits on cosmids of C. elegans.
Consider only hits that have query coverage over 80%.
In your assignment submission, give the names of the C. elegans genes. Also, describe how you came up with your answers. Illustrate that description with the results of the Entrez searches, BLAST searches, and database accesses which you performed. Use "screen dumps" from your web browser to show those results. Have the screen dumps in either PostScript (.ps file) or PDF (.pdf file) form. Marks will be given for the clarity of your description.
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"databases": https://www.ncbi.nlm.nih.gov/guide/all/#databases
"search tools at NCBI": https://www.ncbi.nlm.nih.gov/ncbisearch/
"Entrez Help Document": https://www.ncbi.nlm.nih.gov/books/NBK3837/
"BLAST Help documentation": https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs
Explanation / Answer
Here, we studied the priming of SARS (show SARS Antibodies)-CoV S by elastase and show an important role for residue Thr (show TRH Antibodies)(795) in the S2 domain.
comparison of x-ray structures of alpha1PI complexes with trypsin, PPE, and human neutrophil elastase (show ELANE Antibodies)
the oxygen of the oxopropyl group of the inhibitor is present as an oxygen anion rather than a hydroxyl group, supporting the role of the "oxyanion hole" in stabilizing the tetrahedral intermediate in ELS1 catalysis
Mouse (Murine) Chymotrypsin-Like Elastase Family, Member 1 (CELA1) interaction partners
The stretch-dependent expression and binding kinetics of Cela1 indicate an important role in stretch-dependent remodeling of the peripheral lung during development and regeneration.
defines the temporal-spatial pattern of elastin (show ELN Antibodies) remodeling during lung development, demonstrates an association of this remodeling with chymotrypsin-like elastase 1
Glucocorticoid receptor (show NR3C1 Antibodies)-null lungs included Chi3l3 (show CHI3L3 Antibodies) (11-fold, P < 0.05), a macrophage inflammatory response gene and Ela1 (9.4-fold, P < 0.05), an extracellular matrix remodeling enzyme.
Human Chymotrypsin-Like Elastase Family, Member 1 (CELA1) interaction partners
Inverse association of HbA1c with faecal elastase 1 in people without diabetes suggests that pancreatic exocrine dysfunction might be an early disturbance that develops in parallel with hyperglycemia.
The results of the study suggest that the levels of apelin (show APLN Antibodies), TNF-alpha (show TNF Antibodies) and elastase-1 are important diagnostic markers of CP in patients with type 2 diabetes mellitus.
Results indicate that the extract of the mycelium of Tricholoma matsutake decreased elastase activity in a dose-dependent manner and reduced the levels of matrix metalloproteinase 1 (MMP-1 (show MMP1 Antibodies)).
Mutant genes (CELA1, HSPG2 (show HSPG2 Antibodies), and KCNK5 (show KCNK5 Antibodies)) in Balkan endemic nephropathy patients encode proteins involved in basement membrane/extracellular matrix and vascular tone, tightly connected to process of angiogenesis.
A low value of faecal elastase-1, indicating reduced exocrine pancrease secretion, is strongly correlated with a poor survival in advanced pancreatic cancer.
Hypermethylation of ELA3B (show ELA3B Antibodies) gene promoter were associated with pancreatic cancer.
pancreatic elastase induced proinflammatory effects are mediated by TLR4 (show TLR4 Antibodies) and NF-kappaB (show NFKB1 Antibodies) in human myeloid cells
Parathormone (show PTH Antibodies) levels and Vitamin D metabolism in female patients with various grades of fecal ELA1 deficiency are reported