Describe kinetic proofreading. What would be the effect on kinetic proofreading
ID: 164331 • Letter: D
Question
Describe kinetic proofreading. What would be the effect on kinetic proofreading of a mutation that accelerated the speed of the RNA polymerase?
What would be the effects of a mutation to a bacterial gene that eliminated a string of “U” nucleotides at the end of the gene?
Compare and contrast sigma factor with TBP.
List the three types of eukaryotic RNA polymerases and what type of RNA each one makes.
The 10 and 35 sequences in bacterial promoters are separated by about two turns of the DNA double helix. How would transcription be affected if a deletion were introduced in the promoter region that moved the 35 sequence to the 29 position? Why would this happen?
Explanation / Answer
Answer
Kinetic proofreading is the process that acheives high fidelity. This is also known askinetic amplification. This mechanism allows the enzymes to differentiate between two possible outcomes giving correct and incorrect products with a high accuracy.
The kinetic proof reading is a molecular recognition process and as the speed of the reaction do not change with the existance of the molecules, the kinetic proofreading property is not altered.