If you could answer these i would IMMENSELY appreciate it, please and thank you!
ID: 165826 • Letter: I
Question
If you could answer these i would IMMENSELY appreciate it, please and thank you!!! -- they are a bit difficult and it's for biochemistry, accurate answers really appreciated :)
1. A certain protein, “X” is normally found in the lysosome. I make two versions of X in vitro. One is glycosylated with Mannose-6-PO4 and the other is not. I introduce these two versions of X into the cytoplasm of cells, and follow their localization within the cell. MOST LIKELY predict where the two versions will end up in the cell. Explain.
2. I manage to recreate the glycolytic pathway in vitro using only purified components. I start the pathway by adding ATP that is radiolabeled using radioactive Phosphorus to the in vitro reaction. MOST LIKELY, predict all the molecules that will get radiolabeled when the pathway reaches equilibrium (assuming I am not replenishing the NAD+ present in the reaction). Explain your answer.
3. A deficiency in Biotin could cause a decrease in the liver’s ability to carry out Gluconeogenesis. Is this TRUE / FALSE? Explain your answer.
4. I normally purify a protein from a mixture using an affinity column containing a lectin. This column cannot purify the protein if the mixture is first treated with a glycosidase. If I take the protein mixture and heat it up to first denature the proteins, MOST LIKELY will I still be able to purify my protein using this column (YES/NO)? MOST LIKELY what will the measured specific activity of the protein in this case be (HIGH/MEDIUM/LOW/NONE)? Explain your answer.
Explanation / Answer
M6P is a targeting signal for the lysosome. The lysosome a have M6P receptors that recognise proteins with M6P. Therefore the protein tagged with M6P will most likely end up the the lysosome. Without this marker, the protein will be excreted outside the cell, which is the default path for any protein in the Golgi apparatus.