Please Please Please help me answer this question. Need serious help. M Inbox (6
ID: 172946 • Letter: P
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Please Please Please help me answer this question. Need serious help.
M Inbox (62) sbandaef... X 0 Files X organic chemistry Isc... X Resonance and acid-b... X Introduction to chirallit... X eroblem les https/vosu.instructure.com/courses/12204/files?preview 3179225 90% e Search Practice problems 10.pdf gfp 3. DNA sequences that might act as enhancers (regulatory elements) can be attached to the gene for green fluorescent protein (GFP) and the combined DNA sequence can be re-introduced into an organism. Compared to problem 2, in this case a basal promoter (RNA polll binding site) is included adjacent to the GFP sequences, so that only enhancers are being evaluated If the DNA sequence attached to GFP actually can act as an enhancer, glowing green pigment will be observed in particular regions of the organism. Gene A is expressed in developing nerve cells, muscle cells, and intestinal cells in the fruit fly embryo. The region of DNA around the transcribed region of gene A is depicted below. The pieces of DNA indicated with lines were attached to GFP and tested for the ability to activate gene expression in the fly embryo In piece 5, the "X" indicates a change to a single base pair. Ask me anything 0 Info 408 PMExplanation / Answer
Enhancers are the DNA sequences which can upregulate or downregulate a expression of gene located near or far away from their acting sites. In the given case, an experiment have been conducted to look if the regions adjescent to the GENE-A are able to induce the expression of GFP cloned in vector. In the experiment, 5 clones have been made having different regions of adjescent DNA from the Gene-A.
Now based upon the 5 given pattern of GFP expression of their respective DNA sequences, we can provide the answers for asked questions:
1. For Piece-1 DNA, is acting as enhancer in the muscle cells since the GFP protein is activated in muscle cells only. So for Gene-A, it will also expressed in muscle cells. In the same way, Piece-2 shall be carrying out expresseion of Gene-A in nerve and intestinal cells.
2. Piece-3 and Piece-4 are subpart of Piece-2 DNA. We can see that Piece-3 has no expression in any of the cells/tissues while Piece-4 has same expression as of Piece-2. Hence we can say that Piece-3 has no enhancer activity while Piece-4 which is working in same manner in Piece-2.
3. The mutation (X) present in Piece-5 resulting in loss of enhancer activity in nerve cells only but retains its activity in intestinal cells. First of all it indicates that there were two seperate enhancers for expression activity of Gene-A in Nerve and intestinal cells. Mutation-X has disturbed the enhancer which was responsible for Gene-A expression in Nerve cells thus it will only express in intestinal cells only after having a mutation-X.
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