In a classical genetic analysis, scientists create mutations within or surroundi
ID: 178255 • Letter: I
Question
In a classical genetic analysis, scientists create mutations within or surrounding cloned gene sequences. They then introduce these mutated gene sequences into cell cultures or model organisms and examine the phenotypes. Geneticists are now adding RNA-induced gene silencing to their gene-analysis toolbox.
Describe how you would use RNA-induced gene silencing to study the role of the Drosophila tra gene in sex determination.
Using RNA-induced silencing, one could mutate the tra gene on each stage and study how the process would proceed.
Using RNA-induced silencing, one could specifically delete the tra gene in constant genetic backgrounds.
Using RNA-induced silencing, one could specifically induce mutations in tra premRNA in constant genetic backgrounds
Using RNA-induced silencing, one could specifically alter tra premRNA levels in constant genetic backgrounds.
a.Using RNA-induced silencing, one could mutate the tra gene on each stage and study how the process would proceed.
b.Using RNA-induced silencing, one could specifically delete the tra gene in constant genetic backgrounds.
c.Using RNA-induced silencing, one could specifically induce mutations in tra premRNA in constant genetic backgrounds
d.Using RNA-induced silencing, one could specifically alter tra premRNA levels in constant genetic backgrounds.
Explanation / Answer
RNA induced gene silencing is not heritable so we cannot study effects in each stage so option A holds to be wrong.
In the process of RNA induced gene silencing RNA molecule tends to inhibit the expression of the gene by degrading the mRNA of the specified gene. So in this process the RNA involved in gene silencing tends to alter the level of RNA level by binding to them. Thus, option D holds true.