CHALLENGING PROBLEMS 27. 2 )The following data represent the base compositions o
ID: 186241 • Letter: C
Question
CHALLENGING PROBLEMS 27. 2 )The following data represent the base compositions of double-stranded DNA from two different bacterial spe- cies and their RNA products obtained in experiments conducted in vitro Species (A +T) (A+U) (A+ (G+C) (G+C) (U+ C) 1.30 0.98 G) Bacillus subtilis E. coli 1.36 1.00 1.02 0.80 a. From these data, determine whether the RNA of these species is copied from a single strand or from both strands of the DNA. Draw a diagram to show how you solve this problem. b. How can you tell if the RNA itself is single stranded or double stranded?Explanation / Answer
a. From the given data it cannot be determine that weather both or only one strand is used in the process of transcription. It is only possible when purine to pyrimidine ratio in not unity then you can tell that only one strand is used a template.
b. For RNA to be Double stranded the % of purines to the % of pyrimidines along with their ratio (A+G)/(U+C) would be 1.0. it is clear for E. coli that it is not the case. Because it has the ratio 0.80. whereas in B. subtilis it is 1.02 so we can say it will be double stranded