Pipetting Practice Worksheet Name I be using a P-20 micropipette. a. What is the
ID: 186533 • Letter: P
Question
Pipetting Practice Worksheet Name I be using a P-20 micropipette. a. What is the masimum volume of fhuid that it can hold20 b, what is the minimum volume of nidi' should hold? 2When using a pipetiter, what tem should always be in place fir 3 a. What is the proper procedure when pipetting between different liquids? Explain why b. What are the exceptions to this nule? 4. When using a fresh tip, what should you check for? 5. Why is it important to avoid touching the pointed end of the pipeter? 6. What is the proper way to hold the pipetter when there is fluid in the tip? 7. What is the correct way to push the plunger on the pipetter? do you know you're drawing up an exact amount of fuid with the pipetter? 9 What i s the proper way of holding the tube containing the solution you want to draw up? , and make sure 10 When drawing up fluid, the plunger should always be released that no 11. a When dispensing the liquid, how should you position the tip? nu sde DE Me hiee b. How should you pash the plunger? 12. Afßer dispensing the liquid, when should you let go of the plunger? Explain why POdal 13. How do you remove the tip? ptu55 e bacy woe aming 14. What is a way to avoid contamination if dispensing a reagent into several tubes that contain a different reagent? 15. When dispensing solution into the gel, the plunger should be pushed 16. When holding the pipetter over the gel, the tip should touch the 17 When should you let go of the plunger after dispensing your solution? but not theExplanation / Answer
1. 2.0 - 20 microlitre it can hold. Hence the maximum it can hold is 20microlitre and minimum is 2 microlitre. Units are very important.
2. The autoclaved or sterilized tips should always be put first.
3. When pipetting between different liquids, the tips being used should be changed Everytime while using otherwise it will contaminate the solution.
4. When using a fresh tip one should always check if it is clean or not. And also if it is of proper size.
5. It is not allowed to touch the tip of the pipette because it is the part which comes in contact with the solution, and it can get contaminated.
6. When there is liquid in the tip hold the pipette without pressing the plunger otherwise liquid will flow out.
7. The right way to push the plunger is that one should not press it completely but only to the extent where it pressed easily.
8. One should set the pipette at the right amount and press the plunger easily.
9. While drawing up liquid the pipette should be held straight. If the liquid is in less amount the pipette can be little slanted.
10. Plunger should be released slowly and make sure that no air bubbles are formed.