Please show work, thank you! 14. (Bonus point question): When we prepare PCR mas

Question

Please show work, thank you!

14. (Bonus point question): When we prepare PCR mastermix for amplifying 16S rRNA gene, the final volume 50.0 1 of 1X PCR buffer, for which 10 1 of 5x PCR buffer and 27.7 ul of H2O are added (see figure A. below). If now the final volume is 25.0 of 1X PCR buffer, one should add PCR buffer, and 1 of 5x ul of H20 (see figure B below). PCR Master Mix for 16S rRNA amplification PCR Master Mix for 16S rRNA amplification uL per rxn 10.0 Reagent uL per rxn 10.0 27.7 Reagent DNA (10ng/uL) H2O Primer 27F (20pmol/uL Primer 1492R (20pmol/uL 5X PCR Buffer Tag Polymerase Total Volume DNA (1Ong/ul HO Primer 27F (20pmol/uL Primer 1492R (20pmol/uL 5X PCR Buffer Tag Polymerase 10.0 0.3 50.0 0.3 25.0 B. Total Volume

Explanation / Answer

Ans ) 5 ul of 5X pcr buffer because if

50ul of 1X pcr buffer use 10ul 5X pcr buffer

25ul of of 1X pcr buffer use 5 ul 5X pcr buffer. And therfore 7.7ul water are added so the final volume will became 25ul.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---