I. Use the DNA sequence below, which encodes a prokaryoticgene to answer the fol

Question

I. Use the DNA sequence below, which encodes a prokaryoticgene to answer the following questions. 31 81 131 21 41 ATGAGGAGTT GACACACAAG AGGAGGTAGC AGTATGGGTA TAATCTAATG 51 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 AccccCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT 61 71 91 121 141 a) Write down the corresponding sequences, find them in the sequence aboveand label them: 35 Consensus sequence: 10 Consensus sequence (Pribnow box) Shine-Dalgarno sequence in corresponding mRNA Start (initiation) codon in corresponding mRNA Stop (termination) codon in corresponding mRNA: (label as -35) (label as Pribnow) (label as SD) (label as start) (label as stop) b) Whatregion of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-131 71-119 74-149 54-119 c) What will the sequence be for the protein translated from this mRNA? d) Where is the 3'UTR? Circle one. 9-131 41-119 81-141 87-119 Il. Use the DNA sequence below, which encodes a eukaryotic gene to answer the following questions. 21 31 41 GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC 51 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC 101 GTAGTCTGAT GCCAGTCGAC TGC 61 71 81 91 121 a) Write down the corresponding sequences, find them in the sequence aboveand label them: (label as TATA) TATA box sequence: Polyadenylation signal in corresponding mRNA Kozak's sequence in corresponding mRNA Start (initiation) codon in corresponding mRNA: Stop (termination) codon in corresponding mRNA: (label as Poly-A) (label as Kozak) (label as start) (label as stop) b) Whatregion of the mRNA contains the open reading frame that will be translated into protein 41-101 27-35 51-59 c) Where is the 3 UTR? Circle one. 59-101 51-123 63-101 71-81

Explanation / Answer

I a) -35 consensus sequence - TTGACA (in the above sequence from 9th base to 14th base)

b) 54-119

IIa)

b) 41-101

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