I. Use the DNA sequence below, which encodes a prokaryoticgene to answer the fol
ID: 197731 • Letter: I
Question
I. Use the DNA sequence below, which encodes a prokaryoticgene to answer the following questions. ATGAGGAGTT GACACACAAG AGGAGGTAGC AGTATGGGTA TAATCTAATG 51 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 AcccCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT 21 71 121 31 81 131 41 91 141 61 a) Write down the corresponding sequences, find them in the sequence above and label them: (label as -35) 35 Consensus sequence: 10 Consensus sequence (Pribnow box) Shine-Dalgarno sequence in corresponding mRNA Start (initiation) codon in corresponding mRNA: Stop (termination) codon in corresponding mRNA (label as Pribnow) (label as SD) (label as start) (label as stop) b) Whatregion of this prokaryotic DNA sequence will be transcribed into mRNA? Cirde one. 1-131 71-119 74-149 54-119 c) What will the sequence be for the protein translated from this mRNA? d) Whereis the 3'UTR? Circle one 9-131 41-119 81-141 87-119 Il. Use the DNA sequence below, which encodes a eukaryotic gene to answer the following questions. 21 71 121 31 41 GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC 51 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC 101 GTAGTCTGAT GCCAGTCGAC TGC 61 81 91 a) Write down the corresponding sequences, find them in the sequence above and label them: (label as TATA) TATA box sequence: Polyadenylation signal in corresponding mRNA Kozak's sequence in corresponding mRNA Start (initiation) codon in corresponding mRNA: Stop (termination) codon in corresponding mRNA: (label as Poly-A) (label as Kozak) (label as start) (label as stop) b) Whatregion of the mRNA contains the open reading frame that will be translated into protein? 41-101 27-35 51-59 c) Where is the 3 UTR? Circle one 59-101 51-123 63-101 71-81Explanation / Answer
The consensus sequence TATAAT label at -10 elements.
The consensus sequence TTGACA label at -35 element
UAA, UGA, or UAG stop codon
AUG initiation codon
A ribosomal binding site, Shine-Dalgarno sequence is found in messenger RNA of bacterial as well as archaeal, generally situated around 8 bases upstream of the AUG start codon. The AGGAGG is a six-base consensus sequence.