Please CHOOSE EITHER 7a. Or 7b. to answer. It is okay to write on the back of th
ID: 198993 • Letter: P
Question
Please CHOOSE EITHER 7a. Or 7b. to answer. It is okay to write on the back of the page a. Serum actually contains thousands of proteins and many of these are of interest to people who are studying human medicine and diseases. Most serum proteins are VERY difficult to see by gel electrophoresis without further analysis. The following picture is from an advertisement for a commercial kit that removes some of the most abundant proteins from serum, to people who want to study some of these rarer proteins. the benefit of The pie chart represents the prevalence of some of albumin and antibodies, among ot large number of other proteins that are also present in the serum. the very abumdant proteins in serum, including Based on what you saw with the rabbit serum, and understanding that human is pretty mach the same, why would someone want to remove the abundant proteins from serum before trying to deo experiments with it? Please be as specific as possible. 2-MacrogobuiEntre Protoome lgM 2D Gel All Medium & Low Abundance Proteins Medium & Low Abundance Proteins Oxford Biomedical Research's ProteoMine™ Technology removes > 95% of HAP 2D Gel 7b. Albumin is produced by the liver and there are a lot of medical conditions that prevent proper albumin synthesis so that the serum level is very reduced. Such conditions include liver disease, some forms of cancer and sometimes, old age. Specifically, how might you use something like the serial dilutions and gel electrophoresis that we did in lab to test whether serum from a patient is in the normal range (34 to 55 mg/ml) vs lower than that? Please assume that we have some normal serum samples for comparison. Please be as specific as possible about what you would see on your gel in the case of a patient who has low albumin levels.Explanation / Answer
7a.The aim of the isolation of low abundant proteins from serum is to identify biomarkers which could be useful in disease identification or for medical purposes. Now, the amount of high abundance proteins are mostly of less important in relation to diagnosis and early biomarker discovery. Additionally the higher concentration can mask the low abundance protein. Thus its necessary to remove the high abundance proteins form serum sample before going to any expeiment with the serum sample.