Please explain the answer for these 2 question. The 2nd question was wrong (7) W

Question

Please explain the answer for these 2 question. The 2nd question was wrong

(7) Which of the enzymes from the chart below produce identical sticky ends? Restriction Recognition GATCC Acatc endonuclease sequence Alul AG/CT TCGA G BamHI G/GATCC GATCT BglII A/GATCT AT Clal AT/CGAT EcoRI G/AATTC | HindIII A/AGCTT AAT Pstl CTGCA/G Sacl GAGCT/C Xbal T/CTAGA ETAGA C GAT AG and the (8) The graph on the left shows the titration curve of graph on the right shows the titration curve of 12 - 10 10- pH pH A " " 0.5 1.0 1.5 2.0 2.5 3.0 0.5 1.0 1.5 2.0 2.5 3.0 moles OH-/moles amino acid moles OH-/moles amino acid A) arginine; glutamine (B) glycine; aspartate C) histidine; arginine D) serine; histidine

Explanation / Answer

The slash sign corresponds to where the restriction enzyme cuts the sequence. If after the cut, the hanging sequence is same or not, that is what you are suppose to analyze.

BamHI and EcorI produce same sequence

BamHI                                     EcorI

G| GATCC                           G| AATTC

CCTAG| G                           CTTCC| G

SImilarly BgIII and PstI

ClaI and XbaI

2. C, histidine and arginine. Every amino acid has characteristic titration profile. You can mark the pH values on the y axis and then calculate the PI by which you can identify the amino acid.

Like in first graph there are three curves in the graph, 1.82, 6.0 and 9.17

pI will be 6+9.17/2 =7.59

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