CHM 1360 Winter 2018 Lab 9 Pre-lab worksheet (6 points) name Step 1: Retrieve th
ID: 205209 • Letter: C
Question
CHM 1360 Winter 2018 Lab 9 Pre-lab worksheet (6 points) name Step 1: Retrieve the gene sequence Use an internet browser to go to the Entrez site sponsored by NCBI (National Center for Biotechnology Information), which includes links to many useful databases: Click on the Gene: collected information about gene loci database. In the search box at the top of the window, type in "human beta globin" and hit Search. The first item retrieved should be HBB, Click on this On the page that pops up, you will see a Summary section with some basic information about this gene. You may need to scroll down a bit to answer the next few questions (1) What protein does the HBB gene encode? (2) Look at the first colored diagram under the heading Genomic regions, transcripts, and products How many exons (thick segments) and introns (thin segments) are in the HBB gene? Keep scrolling down until you find the NCBI Reference Sequences (RefSeq) heading. From here you can explore the gene's sequence. Locate the "NG 000007.3" entry under Genomic. Beneath this is an option to Download. Click on the link labeled GenBank. This will bring you to a page of information about the gene (3) On what chromosome is the HBB gene located? Scroll all the way to the bottom to see the DNA sequence. (4) Approximately how many nucleotides make up the beta globin gene? (5) What is the maximum number of codons that this sequence could contain? (6) Can you identify a start codon? If the start codon in mRNA is AUG, what will this codon be in the DNA sequence?Explanation / Answer
Answer 1 beta globin protein
Answer 2 There are 5 exons, and 4 introns.
Answer 3 HBB gene located on chromosome 11.
Answer 4 3043 nucleotides.