CHM 1360 Spring 2016 Lab 3 Protein Concentration Pre-lab worksheet name Megan Ho
ID: 972571 • Letter: C
Question
CHM 1360 Spring 2016 Lab 3 Protein Concentration Pre-lab worksheet name Megan Hovchlo name NIS (I point) Tubes A and B contain different concentrations of a certain molecule. absorbance of 0.238 at 500 nm. Tube B has an absorbance of 0.067 at 500 nm. 1. n molecule. Tube A has an nm. What can you clude? tubes were measured at different wavelengths of light. (b) Tube A allows more light to pass through than Tube B. (c) Tube A has a higher concentration of the molecule. (d) Tube B appears darker than tube A. set of sucrose set of sucrose 2. (I point) Could you use Coomassie Blue to determine the concentrations of a solutions? Explain. London tPeron forces bind fo (I point) The standard curve shown here comes from an experiment that shows how Substance Q affects the activity of a protein that carries out a chemical reaction. A tube containing an unknown amount of Substance Q gave a protein activity measurement of 0.08 reactions per second. Estimate the amount of Substance Q in the tube 3. 0 16 0.14 0.12 proten1 activity 10 (reactions08 protein per second) 0 3865 0.003 0.9394 00 0.1 02 3 04 8 07 0 substance Q (Hg) (1 point) You take 54 L ofa 7.2 gmL solution of BSA and add it to 0.15 mL of water. Wha is the final concentration of BSA? 4.Explanation / Answer
1 since A =ebC
and hence higher the absorbance, higher the concentration( C is correct)
2. In cosmositc blue, the proteins are bound by eletrostatic forcces where as in case of sucrose solution, it is hydrogen bonding
3. since the equation of the best fit is not visible, horizontal line is drawn at 0.08 ( from y- axis) and a vertical line drawn down intersets x axis at 0.41(ug). This is amount of substance.
4.54uL =54*10-6 L =54*10-6*1000 ml = 0.054 ml mass of 7.2 ug/ml, is 7.2*0.054 mg =0.3888 mg
hen 0.15ml water is added, the concentation = 0.3888/0.15 mg/L=2.592 mg/L