II. Phosphofructokinase C) Phosphofructokinase catalyzes the reaction Phosphofru
ID: 211167 • Letter: I
Question
II. Phosphofructokinase C) Phosphofructokinase catalyzes the reaction Phosphofructokinase Mechanism shown here. This enzvme is an important enzyme in respiration, and it is under both positive and negative allosteric regulation. ATP and phosphoenol pyruvate cause the enzyme to act more slowly, and AMP and a different fructose derivative cause the enzyme to act more quickly. As a result there are a total of 6 binding sites on this enzyme; two for the substrates and 4 for the allosteric regulators Looking at the figure and reading what the po above description, what is unusual about the described above? (1) NH2 O-P-O-P-O-P-C 0 0 ATP OH OH H HO HO H ructose-6-phosphate H HO NHz HO H CH2 tose-1,6-bisphosphate o o ADP D) To which site would ATP bind the most easily? Explain (1) E) What sort of amino acid side chains might you expect to find in the binding sites for ATP? (1)Explanation / Answer
approximately of 250 amino acids migh expectedly present in the binding site for ATP