Craig Venter and his colleagues wanted to know if a complete genetic system coul

Question

Craig Venter and his colleagues wanted to know if a complete genetic system could be produced using chemicals and a computer-generated DNA sequence. In 2010, they published an article describing the first self-replicating synthetic bacteria (M. mycoides JCVI-syn1.0) that has a computer sequence as its parent. To verify that they had synthesized a new organism, Venter's team put DNA sequences in M. mycoides JCVI-syn1.0 that spell out secret messages. One DNA sequence is printed out three times below, broken into different triplet reading frames. As in other organisms, open reading frames (ORFs) in this synthetic bacteria start with the codon 5'ATG 3' (written as 5'AUG 3' in the codon table, because it is mRNA that is translated into amino acids). This DNA sequence does not encode any introns, and an entire ORF is encoded in the given sequence. Use the SINGLE LETTER AMINO ACID abbreviations in the codon table given below to uncover the secret message encoded by the ORF. 15 30 45 60 #1 5,-AAA TTT ATC GTT CTG TGT TCT ??? ??? CAA TTG CAC GAC ACA TTG AAA TAA ATT GAG ATC -3, 3'- TTT AAA TAG CAA GAC ACA AGA GAT GAG GTT AAC GTG CTG TGT AAC TTT ATT TAA CTC TAG-5 #4 #2 5,-A AAT TTA TCG TTC TGT GTT CTC TAC TCC AAT TGC ACG ACA CAT TGA AAT AAA TTG AGA TC -3, 3'- T TTA AAT AGC AAG ACA CAA GAG ATG AGG TTA ACG TGC TGT GTA ACT TTA TYT AAC TCT AG-5, #5 #3 5,-AA ATT TAT CGT TCT GTG TTC TCT ACT CCA ATT GCA CGA CAC ATT GAA ATA AAT TGA GAT C-3, 3'- TT TAA ATA GCA AGA CAC AAG AGA TGA GGT TAA CGT GCT GTG TAA CTT TAT TTA ACT CTA G-5, #6 15 30 45 60

Explanation / Answer

Answer

All are same sequence

DNA SEQUENCE number 2: upper strand 40-42 is a start codon
3' TAC ACA GCA CGT TAA CCT CAT CTC TTG TGT CTT GCT ATT TAA 5'

mRNA
   5'AUG UGU CGU GCA AUU GGA GUA GAG AAC ACA GAA CGA UAA AUU 3'

      N-Met-Cys-Arg-Ala-ile-Gly-val-Glu-asn-Thr-glu-Arg-C'

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