In the Ga14-lexA swapping experiment, what was the experiment and what were cont
ID: 222808 • Letter: I
Question
Explanation / Answer
The answer is D;Where they tested the Gal4-lexA hybrid and the controls are Gal4 and LexA missing the DNA binding domain;
For more information refer below.
Principle of domain swap experiment:
A hybrid protein with the DNA binding domain of LexA and the activation domain of the GAL4 protein will activate the modified promoter with the LexA operator or vice versa. This is to show that the AD and the DB domains function independently of each another.
Domain Swap experiment:
Using recombinant DNA technology, the Gal4 transcription activation domain (AD) was fused to the DNA binding (DB) domain of an E.Coli protein called LexA. (LexA is a repressor that binds to a known DNA sequence, the LexAoperator (LexA OP).
These chimeric proteins were introduced into yeast cells with the appropriate LacZ reporter gene constructs.
The results from these experiments showed that the AD and the DB domains function independently of each another.
Similar domain swap experiments are widely used to identify functional domains of regulatory proteins.