Civ) What is the molar ooncentration of a 1 mamL immune globulin eolution? Fig.
ID: 226556 • Letter: C
Question
Civ) What is the molar ooncentration of a 1 mamL immune globulin eolution? Fig. 2 comparison of mtDNA sequence sesss-t01Te of putative Taar position 10100 putative Tsar (arrowed in contrast with sequence from blood samples of relatives 7 Which strand of mitochondrial DNA would result from sequencing with a primer that is top strand sequence (ii) Which strand of mitochondrial DNA would result from sequencing with a primer that is the reverse complement of top strand sequence? (iii) Where are the smaller sequencing products depicted on the chromatograms above? Where are the sequencing products from the bottom ofthe gel depicted on the chromatograms above? (v) Where is the 3 end of the sequence?. (vio In sample 1 at nucleotide 16,169 (arrow), the peak corresponding to cis at 2.5 and the peak position corresponding to T is at 1. What does this mean in terms of relative amount of template with Cat 16,619 compared to Tat position 16,169? 8. Design 15 nucleotide long PcR primers from the beginning and end (use the underlined sequences otthe 3 following sequence (grouped into ten nucleotide blocks), writing the forward and reverse primers in the 5 to direction. GCACGTCGAG CTACGTAAGT ATCGATACGT 5- GCTGTCACAG TCGATCGCAT GCTCGAGCAC CGCGCATTTACGATCGATCA. TTACGATTAC T-3' (1 point) GCTT TACGAC GCAGAT Forward primer: 5 Reverse primer: 5' (i) What is the size of the PCR product amplified from the template shown (include units)?
Explanation / Answer
6. 1. As SDS PAGE is Denaturing gel electrophoresis, it show only 2 bands. One at Top 50kDa band belongs to heavy chain & a bottom 25kDa band belongs to light chain
ii. If non redusing SDS PAGE is used that lack Beta Mercapto Ethanol, which breaks Disulphide bonds, single band of 150kDa appear
Iii. The mass of immunoglobulin is 150kDa or 150000Da or mass = 150000 x 1.67 x 10-21grams. (1 Dalton = 1.67 x 10-21 gms)
15 x 1.67 x 10^-17 gms
25 x 10^-17 grams