Replication is a multiple-steps process that involves many different enzymes. Fo
ID: 226605 • Letter: R
Question
Replication is a multiple-steps process that involves many different enzymes. For each of the following replication step (A-to-I), indicate the enzyme that mediates the process (1 point each). Then, order the steps (from 1 to 9) as they chronologically occur (and as you have been taught in the class) (4 points).
A. Small DNA fragments of ~200bp are synthesized Enzyme: ________________________________________ Order: ___________
B. The DNA helix is further unzipped Enzyme: ________________________________________ Order: ___________
C. A short RNA fragment is synthesized to serve as a primer for the replication of the lagging strand template Enzyme: __________________________________ Order: ___________
D. RNA is replaced with DNA Enzyme: ________________________________________ Order: ___________
E. A short RNA fragment is synthesized to serve as a primer for the replication of the leading strand template Enzyme: ________________________________________ Order: ___________
F. Double Strand DNA is denatured at the origin of replication Enzyme: ________________________________________ Order: ___________
G. DNA fragments are joined together Enzyme: ________________________________________ Order: ___________
H. The leading strand is synthesized Enzyme: ________________________________________ Order: ___________
I. RNA fragments are degraded Enzyme: ________________________________________ Order: _
Explanation / Answer
A. DNA polymerase III
B.Helicase.
C. Primase.
D. DNA polymerase I.
E. Primase.
F. Initiator protiens.
G. Ligase.
H. DNA polymerase III.
I RNAse or DNA polymerase I.
Chronological order;
F, B. E C D A H I G