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Case: A 10 month old child was referred to a lab for testing. The child was very

ID: 238485 • Letter: C

Question

Case: A 10 month old child was referred to a lab for testing. The child was very pale and listless. The following tests were ordered: CBC, reticulocyte count, total serum bilirubin, total serum iron and TIBC.

Results

Patient result

Reference range

WBC

10.5 x10^9/L

RBC

3.5 x 10 ^12/L

3.6 – 5.2

Hemoglobin

5.6 g/dl

10.4 – 15.6

HCT

24%

35 - 51

MCV

68.6 fl

76 - 92

MCH

16 pg

23 - 31

MCHC

23 g/dl

32-36

Platelet count

200 x 10^9/L

Reticulocyte count

0.5%

0.5 – 1.5

Total bilirubin

0.9 mg/dL

0.5 – 1.5

Serum iron

40 ug/dL

50 - 160

TIBC

465 ug/dL

250 - 400

Percent saturation of transferrin

8.6%

20 - 55

The peripheral blood smear had significant anisocytosis, microcytosis, hypochromia and poikilocytosis, with normal platelets.

1. What type of anemia is suggested by the blood smear?

2. What laboratory assays would be of additional value?

3. What is the most probable cause of this anemia?

4. Will the soluble Transferrin receptors be increased or normal?  

Patient result

Reference range

WBC

10.5 x10^9/L

RBC

3.5 x 10 ^12/L

3.6 – 5.2

Hemoglobin

5.6 g/dl

10.4 – 15.6

HCT

24%

35 - 51

MCV

68.6 fl

76 - 92

MCH

16 pg

23 - 31

MCHC

23 g/dl

32-36

Platelet count

200 x 10^9/L

Reticulocyte count

0.5%

0.5 – 1.5

Total bilirubin

0.9 mg/dL

0.5 – 1.5

Serum iron

40 ug/dL

50 - 160

TIBC

465 ug/dL

250 - 400

Percent saturation of transferrin

8.6%

20 - 55

Explanation / Answer

1) It is iron deficiency anaemia

The following are the features of this anaemia

Low haemoglobin
Low ferritin

Low serum iron

Low transferrin.
Raised TIBC.
Low MCV.
Low MCHC or normal.

Peripheral blood smear shows:
Microcytosis and hypochromasia.
There is anisocytosis, poikilocytosis.

2)serum ferritin radioimmunoassay which assess the body stores of iron.

3)cause:

Poor dietary habits which includes drinking only milk in this age group

4) Soluble transferring receptors increase in iron deficiency anaemia.