Reminder: All post labs need to be 1- typed (not handwritten), 2- original (not

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Reminder: All post labs need to be 1- typed (not handwritten), 2- original (not copied from a classmate), 3- answered using complete statements and 4- turned in at the beginning of the lab Post-lab questions PCR and gel electrophoresis Lab Name: Date: Group: T W R 1- Why is PCR typically performed with DNA obtained from 2- What are the necessary reagents to perform PCR? Explain 3- What are STRs? Why are these sequences useful for this 4- Explain what the temperature settings in the thermal cycler a crime scene? Explain. 5 pts the reason why each of these are needed. 15 pts type of analysis? 5 pts are not constant but go from 95 to 52 and then to 72. Indicate what is achieved at each of these temperatures using the correct terminology. 20 pts ?? 5- What explains the movement of DNA through an agarose gel? 5 pts 6- DNA profiles in this experiment require first PCR and then gel electrophoresis. What is the function of each? 5 pts 7- How is an allele ladder used in this experiment? 5 pts 8- How was a DNA profile achieved in this lab? Insert a labeled photograph of your group's gel. Insert a table that summarizes and explains the results (Allele ladder, CS genotype, Suspect 1-4 genotypes) 30pts 10- Does the Crime Scene DNA Sample have a genotype that matches any of the suspects? If so, which one matches? Which suspects were excluded? 5 pts 11- Would the DNA evidence as obtained in this lab be enough proof to incriminate a suspect? Why or why not? 5 pts

Explanation / Answer

1. Generally, the DNA available in a crime scene will be very less and more analysis has to be performed in that DNA. So to have enough DNA sample, PCR is performed for the amplification the sample DNA.

2. Necessary reagents:

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