In the table below, list why each of the groups is important. In other words, br
ID: 253191 • Letter: I
Question
In the table below, list why each of the groups is important. In other words, briefly describe (I sentence) what the control demonstrates or why it is need in order to generate interpretable conclusions from the experiment. Treatment Purpose of the control To make sure that the JM109 bacteria are viable (able to live on growth media) and that they weren't arbitrarily killed by the experimental procedure JM109 (pM1); no abx JM109 (pM:zeo100 This istose that the bacteria that aow or the ue medim afe fesstont to zelo antibioic on JM109 (pMI); amp 100 This is t?See trat the bacteria thatgov n the med vm ampiuale esstart to only ampciin a the lern concertaton ale tetare toonyampicilin the ven conce Haton JM109 (pMI): zeo100 ampl00 s is to check the gfovwth of all bicdei ihose Whrt afe eistat to zelo ard ampic(meanint the baciefial co ngiation has taken ple HY842; no abx The Heigha ?er, a con ow on the rnedium withot an. ont biot C40 check the num be{ af celones pa duced) with???mbioti s To make sure that the HY842 bacteria are resistant to zeo at this particular concentration and are thus able to grow under these conditions. HY842; zeo100 To make sure that the HY842 bacteria are killed by this concentration of ampicillin in the absence of conjugation HY842; amp100 HY842; zeo100 amp100 To check the number ef colonies ploduxed wath the & antilbich meaning f badeial corjuctotion is suctessiul of not JM109 (pM1) + HY842, no abx???Explanation / Answer
This is to make sure that none of the JM109(pM1) and HY842 cells die during the conjugation process. We can compare the viability of both the cells by calculating the difference in colonies of JM109(pM1) and HY842 conjugating cells without antibiotics and JM109(pM1) and HY842 cells individually without antibiotics