Metabolism 1. The formation of acetyl CoA from acetate is an ATP-driven reaction
ID: 257745 • Letter: M
Question
Metabolism 1. The formation of acetyl CoA from acetate is an ATP-driven reaction. Acetate + ATP + CoA-acetylon + AMP + PPi Calculate ??" for this reaction using data given in the table below. Reactions that are reversed result in reversing the sign of the AG value a. b. The PP, formed in this reaction is rapidly hydrolyzed with a AG value of-33.4 kJ mol-1. Cachulate the AG for the overall reaction. What effect does the hydrolysis of PP, have on the formation of scetyl CoA? PP2P 2. Running downhill. Glycolysis molecules of pyruvate with the concomitant synthesis of two (AG ) for this set freactions is-35.6 kJ/mol, whereas the Gibbs free energy change (AG)is 746? 1 Explain why the free-energy release is so much greater under intracellolar conditions than under standard is a series of 10 linked reactions that convert one molecule of glucose into two molecules of ATP. The standard free energy change ain 1-0 3. Define catabolism. What types of enzyme-catalyzed reactions can be considered catabolic? 4. How are metabolic pathways regulated? List the major characteristics of metabolic pathways. Draw a generic 5. In multistep metabolic pathways, enzymes for successive steps may be associated with each other in multienzyme pathway and show a form of regulation possible. complexes or be bound in close proximity on membranes. Explain the major advantage of having enzymes organized in either of these associations. at e 6. What is the direction of each of the following reactions when the reactants are initially present in equimolar amounts (on what side of the equation does the equilibrium reside)? Use the data given in Table below. a. Creatine +ATP Phosphocreatine+ADP b. Glycerol ATPGlycerol-3-phosphate+ ADP c. Pyruvate + ATP ? Phosphoenolpyruvate +ADP d. Glucose + ATPGlucose-6-phosphate + ADP Wh Standard free energies of the hydrolysis of some phosphorylated compounds Compound PPi (pyrophosphate? 2 Pi (inorganic phosphate) Phosphoenolpyruvate 1,3-Bisphosphoglycerate 3-Phosphoglycerate + Pi .29 -61.9 49.4 43.1 30.5 20.9 13.8 9.2 -31 45 ne ? Creatine + Pi ATP-> ADP + Pi Glucose 1-phosphate >Glucose+ Pi Glucose 6 Glycerol 3-phosphate ? Glycerol + Pi Acetyl CoA Acetate +CoASH ATP AMP + PPiExplanation / Answer
3. catabolism is a process in which larger molecules break down into smaller molecules. The enzyme catalyzes reactions that reduced the substrate simpler product are considered catabolic.
4. Metabolic pathways are regulated by enzymatic activity. some of the characteristics of the metabolic pathway are:
It changes the biomolecules in series of steps, not in a single step.
each step is chemically catalyzed by enzymes.
every step has its own specific enzyme.
5. Enzymes associated with each other in a particular arrangement helps in making the process faster with minimum abberation