Choose the gel below that shows the expected bands that would result if the stud
ID: 258057 • Letter: C
Question
Choose the gel below that shows the expected bands that would result if the student completely digested the desired plasmid with Sal I and ran the products out on a gel. (2 marks, all or none)
Select one:
Question 6 Answer saved Marked out of 2.00 Choose the gel below that shows the expected bands that would result if the student completely digested the desired plasmid with Sal land ran the products out on a gel. (2 marks, all or none) Select one: cross out Flag question -Sal i 1 kb pUC18- pCamR- desired ladder 207 GFP plasmid 1 2 3 4. Lanes 8000 bp 7000 bp O A6000 bp_ 5000 bp 4000 bp_ 3000 bp 2000 bp _ 1000 bp __ 100 bp cross out Sal I 1 kb pUC18- pCamR- desired ladder 207 GFP plasmid 1 4 Lanes 8000 bp 7000 bp 6000 bp 5000 bp 4000 bp 3000 bp 2000 bp 1000 bp 100 bp OB. -_Explanation / Answer
Answer:
The desired plasmid if digested with Sal I will produce two different bands because of double digestion. The double digestion with Sal I will produce almost similar length bands because the restriction sites of Sal I are on opposite sides and will thus produce two bands of equal lengths. Also the plasmid's length should accomodate the desired gene to be cloned. Therefore after knowing all the parameters, the option (C) should be the correct option.