Question
Choose the gel below that shows the expected bands that would result it the student completely digested the desired plasmid with Not I and ran the products out on a gel. Remember the desired plasmid is mRFP1 sub cloned into pUC19-207. A biology 207 student sub cloned the mRFP1 gene from pkanR-mRFPl into pUC19-207 using a double digest. They used competent, prototrophic E. coli cells in their translormation protocol and plated the transformation reaction onto medium that contained additional supplements to help them screen and select for their desired transformants. All experimental procedures were followed such as incubation times etc. Mote that mRFP1 encodes for a protein that fluoresces red. Diagrams of the plasmids are below, use them to answer the question that follow on this page. How large is the mRFP1 gene? Express your answer in base pairs.
Explanation / Answer
A