Incorrect Question 1 0/0.5 pts Which of the following needs to be TRUE in order
ID: 260857 • Letter: I
Question
Incorrect Question 1 0/0.5 pts Which of the following needs to be TRUE in order to have E. Coli bacteria glow green under UV light? E. Coli needs to express the GFP gene E.Coli needs to be transformed with a plasmid containing GFP E. Coli naturally have the GFP gene in its genomic DNA E. Coli needs to be transformed with a plasmid containing the ampicillin resistance gene E. Coli transformed with the pGLO plasmid needs to be plated on media with arabinose E.Coli containing the pGLO plasmid needs to be plated on media containing ampicillinExplanation / Answer
1 - E.Coli bacteria grow green under UV light :
E.Coli transfer with pGLO plasmid need to plated on media with amphicillin
E.Coli transfer with pGLO plasmid need to plated on media with arbinose
E.Coli needs to be transferomed with plasmid with GFP
There are several plates contain with various combinations of LB agar, amphicillin, and arabinose.
E.coli is transformed with plasmids that have been modified, and E.coli is injected into the transformation solution, CaCl2.
After two to three days, we discover that the bacteria will only glow in the plate that contains the combination of LB/amp/ara (ara stands for arabinose).
Arabinose is the sugar that activates the production of green fluorescent protein.
In order to make the bacteria glow under the UV light, we need to insert DNA, plasmid, transformed gens and arabinose.
When the ampicillin resistance gene is present it directs the production of an enzyme that blocks the action of the chemical and the bacteria are able to survive.
Bacteria without the plasmid and hence the resistance gene are unable to grow on a plate containing ampicillin in the medium and only the transformants will survive.
The GFP is actually located in discrete spots around the bell margin of the jellyfish and will fluoresce under certain conditions.
When inserted into a plasmid and used for the transformation procedure, the transformed bacteria will express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow green under ultraviolet light.
The mutant form of GFP used in pGREEN makes the bacteria a yellow-green color even in white light
2 - Information for a gene is ....
All are right except the promotor region is must be downstream of gene.
True is : The promoter region is must be upstream of gene.
A gene is a sequence of DNA or RNA which codes for a molecule that has a function.
During gene expression, the DNA is first copied into RNA.
The RNA can be directly functional or be the intermediate template for a protein that performs a function.
The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits.
These genes make up different DNA sequences called genotypes.
Genotypes along with environmental and developmental factors determine what the phenotypes will be.
Most biological traits are under the influence of polygenes (many different genes) as well as gene–environment interactions.
Some genetic traits are instantly visible, such as eye color or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemicalprocesses that constitute life.
3 - Necessary component fot traslation :
- m-RNA
- t-RNA
- Ribosome
- RNA polymerase
In molecular biology and genetics, translation is the process in which ribosomes in the cytoplasm or ER synthesize proteins after the process transcription of DNA to RNA in the cell's nucleus. The entire process is called gene expression.
In translation, messenger RNA (mRNA) is decoded in a ribosome, outside the nucleus, to produce a specific amino acid chain, or polypeptide.
The polypeptide later folds into an active protein and performs its functions in the cell.
The ribosome facilitates decoding by inducing the binding of complementary tRNAanticodon sequences to mRNA codons.
The tRNAs carry specific amino acids that are chained together into a polypeptide as the mRNA passes through and is read by the ribosome.