Preparation of Smeers and Simple Staining Questions 1. Which bacterium is a rod?

Question

Preparation of Smeers and Simple Staining Questions 1. Which bacterium is a rod? 2. Of what value is a simple stain? 3. What is the purpose of fixing the smear? 4. In heat fixing, what would happen if too much heat were applied? Critical Thinking ethylene blue can be prepared as a basic stain or an acidic stain. How would the phi affect the staining of bacteria? C s other than methylene blue be used for direct staining? Briefly explain. 3. Bacteria can be sen withour saining Why then was Koch' recommendation for fxing and staining imporant for microbiology

Explanation / Answer

1. ANS: Bacillus megaterium (rods, chains)

2. ANS Simple stains can be used to determine cell morphology size and arrangement

What is the purpose of fixing the smear?

3. ANS: fixing denatures bacterial enzymes, preventing them from digesting cell parts which can cause the cell to break. It also enhances the adherence of bacteria cells to the slide

4. ANS: It could change or distort the cell

Critical thinking:

1. ANS: Methylene blue chloride dye is used in a simple stain and the pH will determine if the bacteria will have a particular charge.

2. ANS: yes, crystal violet, basic fuchsin and safranin are all dyes that can be used in direct staining because they are cationic with a positive charge

3. ANS: fixing and staining allows the bacteria to be saved and re-examined and shared for study

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