QUESTIONS: 1. 2. 3. 4. What is a standard curve? Why do you need it? Describe a
ID: 274744 • Letter: Q
Question
QUESTIONS: 1. 2. 3. 4. What is a standard curve? Why do you need it? Describe a general procedure to create a standard curve relating one variable to another Name 2 classes of proteins in serum What ion is responsible for complexing with the peptide bonds of the protein in the biuret assay? Looking at standard curves and unknowns what is the significant advantage that the Bio- Rad assay has over the Biuret Assay? What are the potential problems with the Bio-Rad assay? If you get a result of 7mg/ml using the Bio-Rad procedure, should you trust that result? 5.Explanation / Answer
(1)Standard curves represent the relationship between two quantities. It is created by plotting two quantities each on X and Y axis. This is in turn used to determine the unknown quantity.
(2)Creating a standard curve
Begin with known quantities called standards
Relate this known quantity to something we can measure, for e.g absorbance
Graph the relationship on X Y plot to create a standard curve
(3)Albumins, Globulins
(4)Copper ion. The biuret complex combines copper II with the peptide bonds,
(5)Biorad assay can detect from 0.2 to 1.4 mg of protein per mL whereas biuret method requires relatively large quantities of protein (1 - 20 mg protein / mL) for detection. Additionally, biuret method sensitive to a variety of nitrogen-containing substances increasing the likelihood of erroneous results. The Biorad assay is more rapid and less susceptible to interfering substances than biuret method.