Problem 17.14 1 of 4 > A major advance in the 1980s was the development of techn
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Question
Problem 17.14 1 of 4 > A major advance in the 1980s was the development of technology to synthesize short oligonucleotides. This work both facilitated DNA sequencing and led to the advent of the development of PCR. Recently, rapid advances have occurred in the technology to chemically synthesize DNA, and sequences up to 10 kb are now readily produced. As this process becomes more economical, how will it affect the gene-cloning approaches outlined in this chapter? Part A What types of techniques does this new technology have the potential to supplant, and what techniques will not be affected by it? Cloning itself will not be affected, whereas techniques of amplification of cloned DNA are likely to be supplanted Using bacteriophages as cloning vectors will not be affected, whereas the use of plasmids is likely to be supplanted Cloning of genes o unknown sequences will not be affected, whereas cloning of genes of known sequences is Directional cloning will not be affected, whereas cloning of genes using single restriction enzyme is likely to be supplanted. ? a e supplanted Submit Request AnswerExplanation / Answer
option C. cloning of genes of unknown sequences will not be affected, whereas cloning of genes of known sequences is likely to be supplanted.
for known sequences, we will artificially synthesize the gene but for the unknown sequence, we have to take help of cloning.