Study questions: 1. Discuss the purposes of titration. 2. List factors that may
ID: 3506912 • Letter: S
Question
Study questions: 1. Discuss the purposes of titration. 2. List factors that may contribute to inconsistencies between 2 people testing the same sample 3. How is a titer result reported? 4. Why is it necessary to change pipettes between samples? 5. Where is the antigen and where is the antibody in this test? 6. What is the diluent? 7. Clinical Application: If an agglutination test can detect >Smg/L of antibody, what is the titer if of the following dilution? (Hint: Multiply the test sensitivity by the dilution factor) Dilution Agelutination01 Clinical Application: If an antibody level of >45mg/L is indicative of disease, do the above results suggest the person has the disease being tested for? 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:1256 2+ 8. 9 Clinical Application: Assuming the test was performed right and the results are correct, what is one possible reason for the negative result in the 1:1 dilution?Explanation / Answer
1. The purpose of titration is to find of concentration of an unknown sample using concentraion of known sample.
2. Not reading volumes correctly, using contaminated solutions, using incorrect quantity of indicator,
titrating at wrong temperature
3. Titer is the reciprocal value of test serum dilution. It leads to 1+ agglutination of the sample cells.
4. If pipettes are not changed, they carry high chances of making an error in calculation due to presence of little amount of solution in the pipette.
5. They are present in plasma and serum.
6. Saline solution is used as diluent
7. 8. The last solution where agglutination occurs is 1:8 ratio solution. The next one has ration of 1:16. So titer value comes to be 8
8. No because it shows negative result from the 1:16 solution dilution itself.
9. There might have been errors in titration like using contaminated solution, not checking volume correctly etc.