Matching Specific Activity, A260/280, SDS Page Gel, Standard Curve, Bradford Ass

Question

Matching

Specific Activity, A260/280, SDS Page Gel, Standard Curve, Bradford Assay, SDS, 2-ME, BSA, Ponceau S Staining, Coomassie Blue Staining

Matching Specific Activity, A260/280, SDS Page Gel, Standard Curve, Bradford Assay, SDS, 2-ME, BSA, Ponceau S Staining, Coomassie Blue Staining A. None of the below B. Ratio of enzyme activity per total mg of protein C. Principally measures peptide bonds. Tyr, Trp, and polar amino acids D. Bestows equal charge to mass ratio E. Principally measures Tyr, Trp, and Phe while nucleic acids interfere with measurement F. Used to determine protein purity and molecular weight G. Ratio of enzyme amount per total volume H. Used as a comparison to determine an unknown I. Only disrupts hydrogen bonds J. Only disrupts di-sulfide bridges K. Used to determine efficiency of transfer L. Measures total protein amount

Explanation / Answer

B. Specific activity

H. A260/ A280

D. SDS-PAGE gel

H. Standard curve

E. Bardford assay

A. SDS

J. 2-Mercaptoethanol

L. Bovine serum albumin

F. Ponceau S staining

F. Coomassie Blue Staining

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