Question
Imagine a chromatographic column with four plates A sample is introduced and is distributed between the mobile phase and stationary phase How does the column will look like after the third transfer? Which of the following description of selectivity factor alpha is incorrect the ratio of two capacity factors the ratio of distribution coefficients the ratio of two retention times the ratio of two adjusted retention times Selectivity factor alpha indicates the separation between the two peak apexes; however, it is NOT directly indicative of the resolution Which column is the most efficient Plate Height - H - (a)0 1 mm (b) 1 mm(c)0 1 cm (assuming all three columns have fixed column length) (c) (b) all of the above none of the above [(a) (b) and (c)] (a) +
Explanation / Answer
1) the answer is (A)
2) selectivity factor is used to analyze the capacity of chromatographic setup to separate the components
It is also known as separation factor
It is ratio of two capacity factros
3) for high effeciency of factor we take more plate numbers and less plate height
so here the 0.1mm plate height will be most effecient
Answer: E