All question that ask for explanation should be succinct and to the point You MA
ID: 488312 • Letter: A
Question
All question that ask for explanation should be succinct and to the point You MAY lose and to the point. points for too many words, or too many extraneous words! Briefly explain means 10-25 words, and explain means up to 50 words. Patients with diabetes get AlC measurement every three months. The test requires that the HbAlC is separated from Hb. This can be done by ion exchange chromatography Comment on how Hb - Alc is different from Hb. Be brief Hb has a higher-or lower (circle) pI compared with HbAlC. If you are working at pH values above the respective pI values of these systems Hb-or-HBA1C (circle) is more negative. You are asked to make IL of a 220 mM buffer at pH 6.4 A. You choose to use MES (structure shown below pKa = 609) Show the conjugate acid and base structures. conj. acid: conj. base: What are the concentrations of conjugate and conjugate base in the buffer? [conj. acid) = ______ mM [conj base] = __________ mM What is the ionic strength of the buffer you created in part b?Explanation / Answer
2. Preparation of buffer
(a) drawn below are structure for conjugate acid and base
(b) using hendersen-hasselbalck equation,
pH = pKa + log(base/acid)
6.4 = 6.09 + log(base/acid)
(base) = 2.042(acid)
(acid) + (base) = 0.22 M x 1 L = 0.22 mol
(acid) + 2.042(acid) = 0.22 mol
conjugate acid = 0.07 mol/1 = 0.07 M = 70 mM
conjugate base = 0.22 - 0.07 = 0.15 mol/1 = 0.15 M = 150 mM
(c) Ionic strength = 220 mM