Please incorporate a way to measure enzyme activity! 1. Sulfer hot springs are a
ID: 497270 • Letter: P
Question
Please incorporate a way to measure enzyme activity!
1. Sulfer hot springs are a common source of archaebacteria, the third domain of life that biohemically may to be more similar to eukaryotes than bacteria. You have just read research indicating that archaebacteria may be a rich source of a novel family of proteins, the Querall family. These proteins bind to selenium and activate other proteins through an unusual sulfhydryl modification of amines within the cell to repair telomeres, restore replicative ability, and as a result, reverse the aging process. Though they bind selenium, they seem to have a basic pl, in the range of 8.7-9.5. They also tend to be in the size range of the most common archae proteins, ranging from 10kDa to 90 kDa. Eager to begin your study, you've already set up your own aritificial hot spring and have a thriving culture of archaebacteria to sample. However, you now need to isolate the proteins.Explanation / Answer
The temperature of of the test should be relatively hot (higher than 60 C) in order to limit other forms of life like bacteria. Some experiments have made test with 100C but it will depend on the characteristics of the archaebacteria.
There´s no relationship between optimun PH and the isolectricpoint (PI)
In order to separate the proteins by its size I would use chromatography since the range is too wide.