I need help with this please i know its antiport active but its the second part
ID: 52856 • Letter: I
Question
I need help with this please
i know its antiport active but its the second part that is confusing me the most.
3. You have isolated a bacterial transporter that transports Mg2+ and Ca2+ in opposite directions. You produce lipid vesicles that contain this Mg2+-Ca2+ pump as the only membrane protein. The Mg2t Ca2+ pumps are oriented such that Mg2+ is pumped into the vesicle while Ca2+ is pumped out. Each pump transports one Mg2+ ion into the vesicle and one Ca2+ ion out of the vesicle during each pumping cycle and ATP hydrolysis powers this transport, as illustrated below ADP inside ATP outside inside inside inside inside outside inside Is this an example of active or passive transport? (1 point) What type of transporter is this protein? Be as specific as possible. (1 point) Predict what would happen if the solution inside the vesicles contained 75 mM Mg2+ and 75 mM Ca2+ ions, while the solution outside the vesicles contained 75 mM Mg2+ and 75 mM Ca2+ ions as well as 1 mM ATP, and you had a 10,000 fold excess of fluid outside the vesicles compared with the volume inside the vesicles. (4 points) Specifically, be sure to state: a) whether you think there would be net movement of ions and b) if so, how the concentrations of the Mg2+ and Ca2+ ions inside and outside the vesicle would change c) In addition, be sure to state whether you would be creating an electrical gradient, a chemical gradient, or both.Explanation / Answer
The example is for active transport as it utilizes ATP for its pumping and its a antiport as both molecules are pumped in opposite direction.
a) The movement of ions would from inside to outside as the concentration of ions inside is very high compared to the outside due to more of amount fluid outside. So the ions would be diluted outside. Ions will move from higher concentration to lower concentration.
b) Concentration of ions inside will reduce till the concentration on both sides become equal.
c) It would be creating an chemical gradient.