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Please fill in the table, and answer questions 12-14 thoroughly. Thank you! The

ID: 64457 • Letter: P

Question

Please fill in the table, and answer questions 12-14 thoroughly. Thank you! The sequence elements listed in the left column are important for gene expression (some only in eukaryotes). Complete the table. For "Sequence", write in the actual conserved sequence (see example below). For "Position within the gene", write in where the sequence element is found relative to the following gene features: promoter, coding exons, 5' UTR, 3' UTR or introns. Use words like upstream, downstream, 5' and 3' to specify position. Name of the Sequence Proximal Promoter Start codon 5" splice site 3' splice site Stop codon Cleavage and Polyadenylation Sequence GU-rich sequence (requiredGU-rich for cleavage) Poly A tail Sequence i.e.TATA Position within the gene rip an nt 12. Some or all of the sequence elements listed above are found in the sense 13. Some or all of the sequence elements listed above are found in the pre- 14. Some or all of the sequence elements listed above are found in the mRNA strand of the DNA sequence. Which ones (provide names)? mRNA sequence (transcribed region prior to splicing). Which ones (provide names)? sequence (after splicing is complete). Which ones (provide names)?

Explanation / Answer

Name of the sequence Sequence Position within the gene Proximal promoter TATA Upstream Start codon AUG Coding exon 5'splice site GU coding Exon 3'splice site AG intron Stop codon UAG, UAA, UGA 3'UTR Cleavage and polyadenylation sequence AAUAAA 3'UTR GU-rich sequence (required for cleavage) GU-rich 5'UTR Poly A tail AAAAA… 3'UTR