Problem 19.1 What answers would you propose to the following fundamental questio
ID: 84338 • Letter: P
Question
Problem 19.1
What answers would you propose to the following fundamental questions?
Part A
What experimental evidence confirms that we have introduced a useful gene into a transgenic organism and that it performs as we anticipate?
Select the six correct answers.
RT-PCR
RFLP
DNA sequencing
ASO
microarray analysis
PGD
SNP
direct assays of the gene product
chorionic villus sampling
Southern blotting
PCR
amniocentesis
Part B
How can we use DNA analysis to determine that a human fetus has sickle-cell anemia?
Select the four correct answers.
Southern blot
RFLP
ASO testing
PCR
CFTR
allele-specific oligonucleotide detection
Part C
How can DNA microarray analysis be used to identify specific genes that are being expressed in a specific tissue?
How can DNA microarray analysis be used to identify specific genes that are being expressed in a specific tissue?
Microarrays can be used for analyzing proteins in genetically modified plants and animals.
Microarrays can be used for determining functionality of a synthetic genome.
Microarrays can be used as platforms on which to hybridize DNA or RNA from various tissues.
Part D
How are GWAS carried out, and what information do they provide?
How are GWAS carried out, and what information do they provide?
Genome-wide association studies involve scanning the genomes of related individuals with a particular disease and comparing them with the genomes of related individuals who do not have the disease. GWAS attempt to identify genes that influence disease risk, and how they can it transferred to the ofspring.
Genome-wide association studies create the data base for determinig level and frequency of mutations in correlation with the country of residence.
One of the most important part of the genome-wide association studies is creating data of responses people with different genotypes for different medical tresatments.
Genome-wide association studies involve scanning the genomes of thousands of unrelated individuals with a particular disease and comparing them with the genomes of individuals who do not have the disease. GWAS attempt to identify genes that influence disease risk.
Part E
What are some of the technical reasons why gene therapy is difficult to carry out effectively?
Select the three correct answers.
The vector can cause the cell to undergo apoptosis.
A suitable vector must be selected and properly engineered to carry and deliver the desired product on an appropriate schedule.
The vector must be stable yet not trigger an immune response.
The vectors available for use in gene therapy are unable to hold even the smallest of human genes.
Delivering therapeutic genes to the appropriate target cells and producing sufficient levels of the gene product to correct the mutant phenotype remain significant challenges.
Explanation / Answer
Part A
What experimental evidence confirms that we have introduced a useful gene into a transgenic organism and that it performs as we anticipate?six correct answers are as below:
RT-PCR
RFLP
PCR
DNA sequencing
Southern blotting
direct assays of gene product
Part B
How can we use DNA analysis to determine that a human fetus has sickle-cell anemia?
Four correct answers are :
Southern blot
RFLP
PCR
allele-specific oligonucleotide detection
Part C
How can DNA microarray analysis be used to identify specific genes that are being expressed in a specific tissue?
Correct answer is:
Microarrays can be used as platforms on which to hybridize DNA or RNA from various tissues.
Part D
How are GWAS carried out, and what information do they provide? Correct answer is:
Genome-wide association studies involve scanning the genomes of thousands of unrelated individuals with a particular disease and comparing them with the genomes of individuals who do not have the disease. GWAS attempt to identify genes that influence disease risk.
Part E
What are some of the technical reasons why gene therapy is difficult to carry out effectively?
Three correct answers will be :