In your job as a crime scene investigator, you are summoned to investigate a mys
ID: 88102 • Letter: I
Question
In your job as a crime scene investigator, you are summoned to investigate a mysterious death. A male in his early thirties, otherwise apparently healthy, is found dead in his home. There is no indication of foul play; in fact, there is no obvious cause of death at all. Upon closer inspection of the man’s home, you discover several small bits of what appear to be unusual mushrooms on the kitchen counter. Based on this observation, you play a hunch and perform a tox screen on what contents there are in the man’s bladder. Your hunch is rewarded, with the tox screen revealing the presence of alpha amanitin in the man’s urine. From this information, what do you conclude about the cause of death? In formulating your answer, be sure to talk in detail about the process that was affected in the victim. To aid you in this, use the following DNA sequence – discuss what would happen to this sequence under normal circumstances, and show the product(s) that would normally be produced by the affected process under normal circumstances. Discuss specifically what part of the process didn’t happen in this case, and how that led to the man’s death.
5'-TTCGACTCGGTATAAATGCCAGAAGTCCTAGAGACTGTCCTCACTCTCGTGACTAGTGCACCGCTAATGCTTGATGGGGCCCTGCCACAT..-3’
3’-AAGCTGAGCCATATTTACGGTCTTCAGGATCTCTGACAGGAGTGAGAGCACTGATCACGTGGCGATTACGAACTACCCCGGGACGGTGTA..-5’
Explanation / Answer
As the victim found with alpha aminitin, a potent transcription inhibitor of eukaryotes.
So it has must stopped transcription of the given dna sequence.which finally leads in the unavailability of that particular protein.