Quantification Of Total Protein Using Bradford Protein Assay Make a plan on how

Question

Quantification Of Total Protein Using Bradford Protein Assay

Make a plan on how you will prepare BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 50 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured with a micropipettor.

25 micrograms/mL
300 micrograms/mL
600 micrograms/mL
900 micrograms/mL
1200 micrograms/mL

Explanation / Answer

For the first solution the dilution ratio is 25 : 2000 = 1 : 80

So, 100 microliters is diluted to 8.0 mL

For the second solution the dilution ratio is 300 : 2000 = 3 : 20

So, 150 microliters is diluted to 1.0 mL

For the third solution the dilution ratio is 600 : 2000 = 3 : 10

So, 300 microliters is diluted to 1.0 mL

For the fourth solution the dilution ratio is 900 : 2000 = 9 : 20

So, 450 microliters is diluted to 1.0 mL

For the fifth solution the dilution ratio is 1200 : 2000 = 3 : 5

So, 600 microliters is diluted to 1.0 mL

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