Prepare 1L transfer buffer using 10X stock (enough for 2 blots) 10X transfer buf

Question

Prepare 1L transfer buffer using 10X stock (enough for 2 blots) 10X transfer buffer (500mL) 15.15g tris base + 72g glycine Fill to 500mL with water Calculate the molarity of tris in the solution and molarity of glycine, pH of solution is 8.5 Prepare 1L transfer buffer using 10X stock (enough for 2 blots) 10X transfer buffer (500mL) 15.15g tris base + 72g glycine Fill to 500mL with water Calculate the molarity of tris in the solution and molarity of glycine, pH of solution is 8.5 10X transfer buffer (500mL) 15.15g tris base + 72g glycine Fill to 500mL with water Calculate the molarity of tris in the solution and molarity of glycine, pH of solution is 8.5

Explanation / Answer

Mollity of glycine, m = (n/W)X1000

n of glycine = 72/63 = 1.143 mol

theefore , m = (1.143/500)1000 = 2.286 m

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