Please answer the following questions based on techniques used to study gene exp
ID: 92754 • Letter: P
Question
Please answer the following questions based on techniques used to study gene expression.
a. For each of the molecules below, which blot would you use to visualize them?
DNA__________
Protein_____________
b. You are a geneticist working with a family with a child that has micromelia. You know that this child must be homozygous for the mutant copy of the Col2a1 gene. Upon looking further, you find that the child produces less Col2a1 protein than someone who is healthy. What technique did you use and how do you probe for the Col2a1 protein?
Explanation / Answer
a) DNA is visualised using southern blot
Proteins are visualised using western blot
b) Protein gel electrophoresis technique can be used to separate proteins. The Col2a1 protein can be probed with the help of flourescence.