In generating knockout mice what is the purpose of using the TK gene as one of t
ID: 95256 • Letter: I
Question
In generating knockout mice what is the purpose of using the TK gene as one of the markers? A. Making sure that the selected ES cell contains the gene of interest. B. Making sure that the endogenous gene of interest has been replaced by the knockout version of the target gene. C. Both a and b D. Neither a nor b In generating knockout mice what is the purpose of using the TK gene as one of the markers? A. Making sure that the selected ES cell contains the gene of interest. B. Making sure that the endogenous gene of interest has been replaced by the knockout version of the target gene. C. Both a and b D. Neither a nor b A. Making sure that the selected ES cell contains the gene of interest. B. Making sure that the endogenous gene of interest has been replaced by the knockout version of the target gene. C. Both a and b D. Neither a nor bExplanation / Answer
Making sure that the endogenous gene of interest has been replaced by the knockout version of the target gene.
Thymidine kinase is negative selection marker gene which is used to verify if our gene of interest is correctly replaced or not for example mouse cells can be grown ganciclovir but presence of TK acts negative selection marker cause death of mouse by converting ganciclovir to lethal toxin if genes are replaced by vector with TK, they cannot be grown on ganciclovir medium indicating the desired gene is transferred by vector.