Post-Lab Calculations Using the slope of your Beer’s Law plot, calculate the mol
ID: 983577 • Letter: P
Question
Post-Lab Calculations
Using the slope of your Beer’s Law plot, calculate the molar absorptivity, of Allura Red. Molar absorptivities are normally reported in terms of L / mol cm or 1 / M cm. Be sure to convert your units so that your answer is reported in terms of molarity instead of micromolarity (1 uM = 1 x 10-6 M). Show all of your work, specify both the and the value of your wavelenght max, and use appropriate units. (The wavelenght max has to be reported because the molar absorptivity changes with wavelength. Therefore, an molar absorptivity reported without a wavelength is meaningless.)
I am not sure if this is all that is needed to solve this question.
y = 2.4633 x + 0.0789
if not, can you tell me what else is needeed or show the formula on how i would set up to get the answer.
Thank you!!!
Explanation / Answer
Solution :-
Beers law formula
A= E *b*c
so the molar absorptivity (E) is calculated from the graph as
the straight line of the graph gives the equation Y= mx+c
the m= slope which is equal to the molar absorptivity when the path lenght is cm.
so the molar absorptivity from the graph E= 2.4633 M- cm-