Map pling The following table shows where different restriction endonucleases (r

Question

Map pling The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (Cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation Wrepresents adenine or thymine. Enzyme Target sequence Cleavage AATTC 3 5' GAATTC 3' 3' CTTAA 3' CTTAAG 5' EcoRV 5' GATATC 3' 5' GAT ATC 3' 3' CTATAG 5' 3' CTA TAG 5' 5' GGCC 3 5' GG CC 3' 3' CC GG 5' 5' AAGCTT 3' AGCTT 3 3' TTCGA. 3' TTCGAA 5' 5' RG 3 YCCWGGR 5 GR 5' 3' YCCWG Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? a) 5' ATCGA.ACTAGGCC 3' b) 5 AAA GCTTGTGATATC 3' M Hind Hind Incorrect The DNA does RV EcoRI not contain the EcoRV target seguence Hae continued Hael Ecol below

Explanation / Answer

For this you have to find the sequence which is present in the given DNA sequence and matches with the sequence which is cleaved by the restriction enzyme.

A. As you can see in this sequence 5' GGCC 3' is present which is cleaved by the restriction enzyme HaeIII. so the correct option is (D).

B. In this sequece if you notice you will find AAGCTT in the first part of the sequence (2nd base onwards) so one restriction enzyme would be HindIII and in the last part of the sequence you will find GATATC (10th base onwards) so the other restriction enzyme would be EcoRV. so the correct options are (A) & (B).

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