In the experiment above, wild type (WT) or double knockout (DKO) mice were fed (
ID: 148042 • Letter: I
Question
In the experiment above, wild type (WT) or double knockout (DKO) mice were fed (administered) glucose. Some mice were also fed 200 mM NaCl or LiCl, as indicated. After the times shown, blood samples were taken and the amount of glucose in the blood was measured. How could the data be interpreted?
What can be concluded from the data?
A Li+ electrochemical gradient can energize increased glucose transport in DKO mice
In the experiment above, wild type (WT) or double knockout (DKO) mice were fed (administered) amino acids (AA). After the times shown, blood samples were taken and the amount of amino acids in the blood was measured, compared to amount of amino acids in the blood before amino acids administration and plotted as change () in the concentration of amino acids. What can you conclude from this data?
Inclusion of salt in the diet has very little affect on absorption of glucose 400 350 E 300 250 200 O 150 o 100 50 400 350 E 300 ) 250 200 150 o 100 4- WT DKO 50 DKOLiCI I 0 0 15 30 45 60 90 120 Time after administration (min) 0 15 30 45 60 90 120 Time after administration (min)Explanation / Answer
The following can be interpreted from the first set of graphs.
Inclusion of only NaCl increased the absorption of glucose I DKO mice. Notice the line of DKO+NaCl salt, it is higher than the DKO+ LiCl.
The conclusion of the data:
The reduced Na+ electrochemical gradient in DKO mice can be restored by dietary Na+, which energizes increased glucose transport. As the glucose absorption increases on NaCl salt it is evident that Na+ gradient aids glucose uptake and increase blood glucose concentration.
The second set of graphs conclude that the DKO mice absorb less amino acids than WT. It is evident from peaks of graphs which are higher in Wt than DKO