Please explain how you got the answers too. Thanks! Will be produced in the foll
ID: 203820 • Letter: P
Question
Please explain how you got the answers too. Thanks!
Will be produced in the following yeast strains under the conditions noted. Use+to indicate that the GAL enzymes are synthesized at greater than basal levels, and to indicate that the enzymes are not synthesized. NOTE: Gal4ts- a recessive temperature-sensitive allele of Gal4 Galactose absen Galactose present (a) wild-type (b) Gal4/Gal4- (c) Gal3/Gal3 (d) Gal80/Gal80 (e) Gal4ts/ Gal4ts (at the permissive temperature) (f) Gal4ts/ Gal4ts (at the restrictive temperature) (g) Gal4'/Gal4ts (at the restrictive temperature)Explanation / Answer
Galactose absent Galactose present
Wild type - +
Gal 4-/Gal4- - -
Gal 3-/Gal3- - -
Gal 80-/Gal80- - -
Gal 4ts/Gal4ts - +
Gal 4ts/Gal4ts - -
Gal4+/Gal4ts - +
Wild type strain have Gal4+ gene which induce synthesis of galactose and activates transcription.
The negative strain of Gal4- gene presence cannot induce synthesis of galactose and doesn't activate transcription in the presence and absents of galactose.
The nagative strain of Gal 3gene(The reactive Gal3p converts galactose to the inducer activates transcription) doesn't activate transcription
The negative strain of Gal80- gene cannot activate transcription as active Gal80+ help convert galactose to inducer and move on to GAL4p+.
The temperature sensitive Gal4ts at permissive temperature can synthesis Gal enzyme.It can toerate temperature upto 18 degree Celcius.
The temperature sensitive Gal4ts at restricted temperature like 30 degree celsius cannot synthesis GAL enzyme.
The Gal 4+ and Gal4ts gene allow yeast to grow in presence of galactose in restrixtive temperature.As Gal4+ is dominant over GAL4ts.