I need help with this please. 26. Add 10 drops of 1.0 M NaOH and 9.5 m 3. Why is
ID: 204203 • Letter: I
Question
I need help with this please.
26. Add 10 drops of 1.0 M NaOH and 9.5 m 3. Why is it necessary to rinse the bags of dH:O to the bag. Seal the open end of the bag by folding and tying as outlined in step 15. Rinse the bag with dH O and place the bag into beaker 6. before you place them in their respective beakers? 4. When is a change in the color of 5. State the location of the color change in 6. What indicator in each series accounts for 7. Were the effectors of the color changes 27, Record the time and ambient temperature phenolphthalein observed? Starch? in your laboratory notebook. each beaker 28. Draw each beaker and the color of the contents, including the bag, in your laboratory notebook. the color change? 29. Label each beaker to properly reflect its contents. This is the Before appearance. added to the locations in which you saw the color changes? If not, why not? C Collecting your Data 8. In which locations were the concentrations 1. Wait 30 45 minutes and record the time and ambient temperature in your of the reagents the highest? What can you conclude then about the diffusion of reagents in each series of beakers? laboratory notebook. 2. Draw each beaker and the color of the contents, including the bag, in your 9. How does this exercise approximate the of materials through the plasma membrane or other cellular membranes? laboratory notebook 10. Since it is not possible to observe the actual osmosis of the water molecules, can you suggest a test to determine whether or 3. Label each beaker to properly reflect its contents. This is the After appearance. not osmosis has occurred? Near the end of the lab period, the instructor will review the chemistry of iodine and starclh and phenolphthalein and sodium hydroxide, and discuss the results of the experiment. GENERAL REFERENCES Do not dispose of any of the results until all of the students in your section have observed them. Alberts, B., A. Johnson, J Roberts, and P. Walter. 2002. Molecular Biology of the Cell, 4h Ed. New York, NY: Garland Science, Inc. Becker, W. M., L. J. Kleinsmith, and J. Hardin. 2005. The World of the Cell, 6 Ed. San Questions Eamim, CA: Benjamin/Cummings Publishing Why is it necessary to thoroughly wash all glassware before beginning this exercise? I. Company Coyne, G. S. 1997. The Laboratory Companion: A Practical Guide to Materials, Equipment, and 2. Why is it necessary to keep the dialysis tubing moist during the beginning of the Technique. New York, NY: John Wiley & Sons, nc Exercise 7: Diffusion through a SimulatedExplanation / Answer
1.Ans-
Washing all glassware is necessary before the experiment to remove the bound metals such as iron and probably magnesium. So, acid washing and rinsing of glassware are required to remove these metals.
2.Ans-
Dialysis tubing is used to clean up and processing of samples like proteins, DNA, and blood or serum. During the beginning of the procedure, it is necessary to keep the dialysis tubing moist because in a dry state they become fragile and break
3.Ans-
It is necessary to rinse the starch and glucose that spilled on the bags so that the water remains distilled in beakers at the beginning of the experiment
4.Ans-
Phenolphthalein used as a pH indicator to determine whether a substance is an acid or a base. It changes its color in basic solution. It is colorless in acidic pH and turns into pink in basic pH