Please explain in detail how to get this answer. Thank you! 6. (6 points)) You p

Question

Please explain in detail how to get this answer. Thank you!

6. (6 points)) You perform a co-transduction experiment with leu+ lac+ arg- mant donor and leu- lac- arg+ man- recipient. On master plate 1, you select for 1000 leut transductants. By screening, you find 110 colonies are lact, 301 are man+, and 999 are argt. On master plate 2, you select for 1000 lac+ transductants. By screening, you find 108 colonies are leu+, 299 are mant, and 701 are argt. Circle ALL of the following orders which may be possible given the above results. (All or nothing) a. leu---lac--man--arg c. man---leu--lac- arg e. arg man---leu---lac g. arg-leu-lac-man b. lac---arg---man---leu d. arg---lac--man--leu f. leu-lac-arg.--man h. None of the above

Explanation / Answer

Ans. to study the cotransduction we need to study the transduction frequencies.If the transduction frequency is low then the distance between the two markers is more that is they are inversely proportional.In the case of leu+ transductants we see the frequency percentage for

lac= 11% (110/1000 *100)

man= 30.1%

arg= 99.9%

here percentage is calculated by taking no of marker plates divided by 1000 the whole into 100

According to this arg is at very close distance to leu and lac is very far from leu

In the case of lac+ transductants we see that

leu= 10.8%

man=29.9%

arg= 70.1%

So here we can say that lac and arg are close to each other and lac and leu are far .

Now there are two conditions leu and arg should be close to each other and lac leu should be far apart

the order should be leu---arg---man--lac

The order in b can somewhat match because lac and leu are far apart and arg can be close to any one lac or leu but none of the above can also be true.

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