I have to transcribe the following: 1. Transcribe the following sequence. You ne

Question

I have to transcribe the following:

1. Transcribe the following sequence. You need to show both the 3’ to 5’ and 5’ to 3 sequences (3 points) Hb A: AGT AAC GGC AGA CTT CTC AGG AGT CAG GTG CAC CAT

2. Translate your new RNA sequence using the genetic code. Remember that when determining your amino acid sequence, the RNA sequence is read from 5' to 3' (4 points)

3. Transcribe the following DNA sequence ( 3 points) Hb S: AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CAT

4. Translate your Hb S transcript (again remember it is read from 5’ to 3’) (4 points)

I don't want to just know the answer but how to get the answers. If you could provide links that could show me why they are that way it would be great. I am trying to use my chart but I don't have a "T" there and don't know why it should be UCA for #1.

I have also found the DNA codon table at this website https://en.wikipedia.org/wiki/DNA_codon_table and was wondering how accurate it is. I know to find the first letter on the left, the second letter on the top and to intersect them but when do I know where to find the final letter and which one to use.

Thank you in advance.

Second letter Phe UCU UAU Tyr UGC UGCFCys Leu UCA Ser Leu ucoG UAA Stop UGA Stop A UAG Stop UGG Trp G CCA Pro CAc His CGU CCC Pro CAA GIn CGG CCG CUU CUA FLeu CCC CUG C CUA AUU AUC lle ACC AUA AUG Met ACG ACU Ser ACA Thr AAcAsn AGU AAG FLys AGA AGG Arg A GUA Val GCC GUG GCU GCC Ala GAA Glu GGG G GUA GCG

Explanation / Answer

Hb A

DNA               5' AGT AAC GGC AGA CTT CTC AGG AGT CAG GTG CAC CAT 3' ( non-template strand)

              3' TCA TTG CCG TCT GAA GAG TCC TCA GTC CAC GTG GTA 5' ( template strand)

RNA           5' AGU AAC GGC AGA CUU CUC AGG AGU CAG GUG CAC CAU3' ( sequence is same as non templete strand, only T is replaced by U)

Protein        SNGRLLRSQVHH

   Ser-Asn-gly-arg-leu-leu-arg-ser-glu-val-his-his

HbS:   5' AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CAT 3' ( non-template)

          3' TCA TTG CCG TCT GAA GAG GTG TCC TCA GTC CAC GTG GTA 5' ( template)

RNA 5' AGU AAC GGC AGA CUU CUC CAC AGG AGU CAG GUG CAC CAU 3'

protein         SNGRLLHRSQVHH

               ser-asn-gly-arg-leu-leu-his-arg-ser-glu-val-his-his

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