Subject: Microbiology Serial dilution of 10mL of saline mixed with 1 tsp of soil
ID: 264549 • Letter: S
Question
Subject: Microbiology
Serial dilution of 10mL of saline mixed with 1 tsp of soil sample was made. labeled each conical tube "Normal" 10%, 1%, 0.1%, and 0.01%. Labeled petri dishes to match. Labeled another set of other conical tubes that were Heated 10%, 1%, 0.1%, and 0.01%. Labeled petri dishes to match. 9mL saline diluted into each tube. Serial dilutions were applied/spread to respective petri plates. Incubated both sets of plates in a warm location.
Colonies from both sets of plates, Heated and Normal, were transferred to 3 circles on 2 microscopic slides (Heated slide and Normal slide). 1 drop of H2O2 was appliedto each circle to observe the formation of bubbles (effervescence).
Which treatment condition produced more catalase positive bacteria? What can explain the difference in the number of catalase positive bacteria?
Write out the reaction that is catalyzed by the enzyme catalase. Why is this enzyme important?
Explanation / Answer
Subject: Microbiology Serial dilution of 10mL of saline mixed with 1 tsp of soil